Composite

Part:BBa_K1660007:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)


PompR-6N-gp35-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 136
    Illegal NheI site found at 333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 607
    Illegal XhoI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1246
    Illegal NgoMIV site found at 1333
    Illegal AgeI site found at 383
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1441


Design Notes

we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.


Source

It comes from the register.

References